Th game, which kicks off at 12.45pm, will be show live on Sky TV and will be played in front of a massive 51,000 sell-out crowd.
The Shielfielders go to Glasgow on the final day of the season knowing that nothing short of a draw, or at the very least a low-scoring defeat will be good enough to earn them a top four finish.
With just one game to go, Berwick sit in fourth place in the table three points ahead of both Elgin and Montrose.
Elgin, with an inferior eight goal difference, are at home to East Stirling, whilst Montrose, 15 goals adrift, face Annan.
The maths are quite clear.
A win or a draw at Ibrox would put Berwick out of reach.
Defeat would need to be by a margin whereby most probably Elgin could not overtake them.
“It’s in our hands,” admitted manager Ian Little.
“The play-offs have been our target all season and it would have been nice to have it all sorted out before going to face Rangers at Ibrox.
“That in itself will put pressure on the players, but hopefully they are big enough to stand up to the pressure and see it through.”
Little was disappointed at not securing fourth spot on Saturday when they went down to Annan, but he said:”We have a second chance and we have to take it.”
Berwick chairman Brian Porteous said the situation was not how he would have planned it but he is grateful they are still in with a chance.
“It will be nervous, there is no question about that,” he said.
“Ideally we would have liked to have wrapped it up before the last game of the season, but that is better than not having a chance at all.
“I think if we are being honest, at the start of the season, when Rangers came into our league, we probably all knew we were only playing for the play-offs, and that’s exactly how it has panned out.
“There will be pressure on everyone at Ibrox, I am certain of that, but hopefully we can do what we have to do.
“Getting into the play-offs would be a tremendous boost for us, because if nothing else it would give us the chance of potentially winning through and getting another two home games against Rangers next season which are financially very appealing.”
Berwick will travel to Ibrox without the services of midfield play-maker Lee Currie who was unfortunately sent off for two bookable offences against Annan.
However, they will be able to call on captain Chris Townsley, who thought he might miss the game if a red card appeal originally scheduled for May 2 was rejected.
The appeal has now been deferred for a further week until May 9 which means he can play.#
Should Berwick make the play-offs they will be home to either East Fife or Stranraer in the first leg of the semi-finals next Wednesday (May 8) with the second leg on Saturday, May 11.