The temporary rules implemented on March 26 must be reviewed every three weeks and new amendments have now been implemented.
The two metre distance rule must be observed by all businesses which remain open, with failure leading to fines or prosecution.
Livestock markets and money advice services can continue to operate, while hotels and other accommodation providers can take bookings online and by phone for future dates. It has also been confirmed that burial grounds can remain open.
Constitutional relations secretary Michael Russell said: “I would like to thank people and businesses across Scotland who have been following social distancing measures over the last three weeks and helping to slow the spread of Covid-19 and save lives.
“From the outset, we have said these regulations are temporary and will be kept under review. After careful consideration, we have decided that it is necessary for these regulations to continue for the protection of public health.
“We have also made some minor amendments to the regulations to enhance their operation and social distancing for businesses, as well as making it more explicit that services relating to livestock, money, and online holiday bookings can remain open.
“The restrictions are tough and have had an impact on everyone’s lives but they are necessary to protect public health and our NHS, and we will continue to keep them under review.”